Abstrakt

Validated stability indicating UPLC method for the simultaneous determination of famotidine and ibuprofen in combined dosage form

P.Venkata Rao, B.Ravi Kumar, M.Ravi Kumar, Dantu Durga Rao

A simple, precise and accurate stability indicating ultra-performace liquid chromatography (UPLC) method was developed and validated for the simulataneous quantitative determination of Famotidine and Ibuprofen in the presence of degradation products. The separation achieved onAcquity UPLC columnwith simple gradientmethod. The mobile phaseAcontains a mixture of pH 6.0 sodiumacetate buffer and methanol in the ratio of 80:20 (v/v) and mobile phase B contains a mixture of pH 6.0 sodium acetate buffer andMehtanol in the ratio of 30:70 (v/v). The peaks were monitored at 260 nm wavelength. This method was validated for accuracy, precision, linearity, and robustness. The method was also found to be stability indicating. Famotidine was found to degrade significantly in oxidative, acid and base stress conditions. The degradation products were well resolved from main peak of Famotidine and Ibuprofen, thus proved the stability indicating power of the method.