Abstrakt

Simultaneous determination of pipenzolate bromide and phenobarbitone in pharmaceutical preparations by HPLC method

Nisreen F.Abo-Talib, Mohamed R.El-Ghobashy

Abinarymixture of pipenzlate bromide and phenobarbitone was determined byHPLC method using 0.05Mammoniumdihydrogen phosphate/acetonitrile/ methanol (7:12:1, by volume) as themobile phase withUVdetection at 220 nm over concentration ranges of 10-90 µg. ml-1 and 1-80µg. ml-1 with mean percentage accuracies 99.90 ± 0.62 and 100.30 ± 0.92 for pipenzlate bromide and phenobarbitone, respectively. The suggested procedure was checked using laboratory prepared mixtures and was successfully applied for the analysis of their pharmaceutical preparation. The method retained its accuracy and precision when applying the standard addition technique. The results obtained by applying the proposed method were statistically analyzed and compared with those obtained by the manufacturer method.