Abstrakt

Protective Effect of Selenium against Oxidative Stress during Ovariectomy and Ovariectomy-Cadmium Exposure on Blood Parameters and Antioxidant Enzymes (Peroxidase and catalase) Activities in Female Rats

Talib Hussen Ali, Ahmed Baker Ali and Heyam Natheer Matti

Ovariectomized (Ovx) sexually mature adult female rats were exposed to sub chronic treatment of cadmium chloride (CdCl2) at a dose of 6 mg/kg/ body weight/rat then, were orally gavaged daily with 2ml of the treatment for a period of 28 days resulting, significant reduction in Erythrocyte count (RBCs), hematocrit percentage (Hct%) andHemoglobin value (Hgb),While mean corpuscles hemoglobin concentration (MCHC) was increased. Moreover, hepatic and renal antioxidant enzymes activities, peroxidase and catalase (CAT) were decreased significantly in comparison with the control. The consequences of ovariectomy operation of female rats exhibited almost the same values of all of the above mentioned Ovx-Cd treatment parameters. Both the experimental rat groups (Ovx and Ovx-Cd) were gavaged orally with a 2 ml solution of the dietary supplementation (seleniumchloride SeCl2) individually or in combinationwith CdCl2 at a dose of 6 mg/kg/b.w/rat, resulting recovery effects. Blood parameters and antioxidant enzymatic activities were almost restored to control level except, for peroxidase activity that responded differently in both types of treatment. The present study revealed significant protective action of selenium on the toxicity induced by Ovx or Ovx-Cd in female rats, especially when selenium was subsequently supplemented to both treatments.