Abstrakt

Callus formation and isolation of cyclodecane from tissue culture of Apium graveolens L.

Seyed Mehdi Razavi, Hadigheh Hejabi

Nowadays, isolation of useful natural products from plant callus culture has been used as a modern biotechnological technique to obtain those useful chemicals. Apium graveolens (Apiaceae) is a well known edible herb was known to produce a variety of plant secondary metabolites such as flavonoids and coumarins. In this study, we focused on induction of callus tissue of the plant seedling that may be used as a source of different metabolites. The seeds of the plants after sterilling were cultured in a petri dishes line with MS medium. After emergence of seedling, epicotyl segments were transferred to another MS cultures with contain different combination of plant hormones, kin and 2,4-D. The petri dished incubated in a growth chamber at 25ºC and certain photoperiod Weight of produced callusweremeasured for all treatments. On the other hand, n-hexan extract of dried callus was obtained by a soxhelet apparatus and were analyzed using thin layer chromatography technique (TLC) to afford a colorless oily substance. The structure of isolated compound was elucidated by spectroscopic methods such as IR, UV, Mass and 13C and 1H NMR. Our results indicated that although callus induction take place inMSmedium without phyto hormones, maximum callus production induced at MS mediumwith 2, 4-D (4mg/L) and kinetin (2mg/L). The structure of isolated natural product from the callus was determined as cyclodecane. It was be concluded that callus tissue of Apium graveolens can be source for production of cyclodecane.