Abstrakt

Application of 3-methyl benzothiazoline-2-one hydrazone as achromogen for the spectrophotometric determination of oriental fruit fly's synthetic attractant

Ezzat M.Abdel-Moety, Hayam M.Lotfy, Nasr S.Khalil, Yasmine Rostom


A simple, rapid and sensitive colorimetric method for quantitative determination of methyl eugenol (ME), a synthetic attractant for oriental fruit fly (OFF), in pure formand in commercial preparations is described. Oxidative coupling ofMEwith 3-methyl benzothiazoline-2-one hydrazone (MBTH) in presence of ferric chloride produces a blue colored product, which absorbs maximally at 619 nm. The color is linear in the range 2.5-10gmL-1,withmean percentage recovery of 100.010.58 and correlation coefficient of 0.9998 (n=7). Various parameters affecting the reaction pathway have been optimized and the method could be successfully applied to determine ME in both pure form and in the commercial preparation without interferences fromexcepients and diluents. Themethod needs neither extraction nor heating. The obtained results were in good statistically agreement with those obtained by applying a reported method. Limit of detection (LOD) and limit of quantitation (LOQ) are reported.


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