Abstrakt

A simple and sensitive RP-HPLC method for simultaneous analysis of nabumetone and paracetamol in pharmaceutical formulations

S.S.Ranher, S.V.Gandhi, P.B.Deshpande, B.B.Gandhi, V.V.Mhatre

Nabumetone is a non-steroidal anti-inflammatory drug used to treat pain or inflammation caused by arthritis and Paracetamol is analgesic and antipyretic drug. This paper describes a simple, accurate and sensitive highperformance liquid chromatographic method for simultaneous quantification of these compounds as the bulk drug and in tablet dosage forms. RPHPLC separation and quantition of drugs was carried out on Jasco HPLC systemwithHypersil C18 column (250mmï‚´4.6mm) using amobile phase of acetonitrile and 0.05%aqueous acetic acid (70:30, v/v) at flowrate of 1ml/ min using Naproxen sodium as internal standard and detection using PDA detector was carried out at 238 nm. The method was validated for specificity, linearity, precision, accuracy, limit of detection, limit of quantification. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. The accuracy of the method was assessed by evaluation of linearity (5-25g/ml for both Nabumetone and Paracetamol), precision (intra-day RSD 0.1097 % and inter-day RSD 0.9899 % for Nabumetone and intra-day RSD 0.0550%and inter-day RSD 1.1511%for Paracetamol), and accuracy (99.07-100.12 ± 0.977 % for Nabumetone and 100.82 ± 0.660 % for Paracetamol) in accordance with ICH guidelines.